منابع مشابه
Narrowing of protein NMR spectral lines broadened by chemical exchange.
Broadening of spectral lines is a signature of chemical exchange phenomena on microsecond to millisecond time scales but has deleterious effects on spectral resolution and sensitivity. A multipulse method based on chemical shift scaling that reduces chemical exchange broadening during frequency-encoding periods of liquid-state multidimensional NMR experiments is described. The proposed scheme u...
متن کاملTheory of chemical exchange in zero field NMR : Two - site flips
With conventional NMR of polycrystalline solids, broad and often featureless spectra are frequently obtained and the effects of some molecular motions are not readily observed. 1 The resolution and sensitivity of nuclear quadrupole resonance (NQR) and broadline NMR is often so low that only the rigid and rapid motional regimes can be studied. In the intermediate region, where the exchange rate ...
متن کاملAuto-FACE: An NMR Based Binding Site Mapping Program for Fast Chemical Exchange Protein-Ligand Systems
BACKGROUND Nuclear Magnetic Resonance (NMR) spectroscopy offers a variety of experiments to study protein-ligand interactions at atomic resolution. Among these experiments, 15N Heteronuclear Single Quantum Correlation (HSQC)experiment is simple, less time consuming and highly informative in mapping the binding site of the ligand. The interpretation of 15N HSQC becomes ambiguous when the chemica...
متن کاملThe Static Magnetic Field Dependence of Chemical Exchange Linebroadening Defines the NMR Chemical Shift Time Scale
The static magnetic field dependence of chemical exchange linebroadening in NMR spectroscopy is investigated theoretically and experimentally. Two-site exchange (A / B) is considered with site A more highly populated than site B (pa > pb), a shift difference between sites equal to ∆ω, and an exchange rate constant given by kex. The exchange contribution to the transverse relaxation rate constan...
متن کاملNoninvasive Measurements of Glycogen in Perfused Mouse Livers Using Chemical Exchange Saturation Transfer NMR and Comparison to 13C NMR Spectroscopy
Liver glycogen represents an important physiological form of energy storage. It plays a key role in the regulation of blood glucose concentrations, and dysregulations in hepatic glycogen metabolism are linked to many diseases including diabetes and insulin resistance. In this work, we develop, optimize, and validate a noninvasive protocol to measure glycogen levels in isolated perfused mouse li...
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ژورنال
عنوان ژورنال: Pure and Applied Chemistry
سال: 1972
ISSN: 1365-3075,0033-4545
DOI: 10.1351/pac197232010009